Human Gastric Biomarkers

Human Extracellular Matrix Components (ECM)

Gastrin-17

Amidated gastrin-17 is a biologically and physiologically important molecule among the family of the gastrin peptides. Amidated gastrin-17 is the most powerful gastrin peptide in the feedback control mechanism of the acid secretion and output from the parietal cells in the gastric corpus. This peptideis solely synthetized and secreted into the circulation by the so called G cells (“gastrin cells”) in the gastric antrum. A positive immunohistochemical reaction for gastrin-17 is a sign of a highly differentiated gastrin-secreting cell and normal antral mucosa. In atrophic gastritis, the normal antral (pyloric) glands and gastrin-17 synthetizing cells disappear. Metaplastic pyloric glands (pseudopyloric metaplasia) do not contain immunopositive gastrin-17 cells.

Pepsinogen I

Pepsinogen I is a group of precursor molecules for pepsin. These proteins are solely synthetized and secreted into gastric lumen by chief (pepsin) cells and mucous neck cells in the gastric corpus (oxyntic mucosa). In atrophic corpus gastritis these cells disappear resulting in a decrease of the serum level of pepsinogen I and in a reduction of the number of pepsinogen I positive cells in gastric biopsies. The presence of positive immunostaining for pepsinogen I is a highly reliable sign for the acid-secreting oxyntic glands. In gastric heterotopia of the duodenal bulb, but not in gastric metaplasia, the oxyntic-type glands give a positive immunohistochemical reaction for pepsinogen I.

Pepsinogen II

Pepsinogen II is a group of precursor molecules for pepsin. These proteins are secreted into the gastric lumen by the pyloric glands of the gastric antrum and also by the chief and neck cells of the gastric corpus (oxyntic mucosa). Negative immunohistochemical reaction for pepsinogen I (right) but positive reaction for pepsinogen II (left) is a typical sign of the antral mucosa and, in the presence of atrophic gastritis, this staining pattern indicates that the positive glands and cells are metaplastic and “pyloric” in differentiation (so called pseudopyloric metaplasia).

Monoclonal antibody ton PEPSINOGEN I Clone 4C6.1 (IgG₁)100 mg immunoglobulin in 1 ml PBS solution containing 1.0% (w/v) BSA and 0.09 % (w/v) sodium azide.

The product is for research use only. The performance characteristics of this product have not been published. Use in human clinical diagnosis is the responsability of the user. This product should be stored at +2…+8 °C.

Monoclonal antibody to pepsinogen I is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. Pepsinogen I purified from human tissue was used as an immunogen.

Specificity
The antibody is specific to human pepsinogen I and has no crossreactivity to human pepsinogen II.

Applications
Immunohistochemical stainings, Western blotting and enzyme-immunoassays.

Monoclonal antibody to PEPSINOGEN II Clone L10CC10 (IgG₁)

100 mg immunoglobulin in 1 ml PBS solution containing 1.0% (w/v) BSA and 0.09 % (w/v) sodium azide.The product is for research use only. The performance characteristics of this product have not been established. Use in human clinical diagnosis is the responsibility of the user. This product should be stored at +2…+8°C.

Monoclonal antibody to pepsinogen II is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. Pepsinogen II purified from human tissue was used as an immunogen.

Specificity
The antibody is specific to human pepsinogen II with no crossreactivity to human pepsinogen I.

Applications
Immunohistochemical stainings, Western blotting and enzyme-immunoassays.

Monoclonal antibody to GASTRIN-17 Clone G52C7.1 (IgG₁)

100 mg immunoglobulin in 1 ml PBS solution containing 1.0% (w/v) BSA and 0.09 % (w/v) sodium azide.The product is for research use only. The performance characteristics of this product have not been established. Use in human clinical diagnosis is the responsibility of the user. This product should be stored at +2…+8 °C.

Monoclonal antibody to gastrin-17 is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. Synthetic (modified) gastrin-17 coupled to thyroglobulin was used as an immunogen.

Specificity
The antibody is specific to gastrin-17 with no crossreactivity to gastrin-34 or cholecystokinin.

Applications
Immunohistochemical stainings, enzyme-immunoassays