Human Cytoskeletal Polypeptides

The cytoplasmic cytoskeleton determines cell organization, shape and adhesion among other functions. Furthermore, the cell type-specific expression of intermediate filaments allows determination of the origin of many, otherwise unspecific tumours. Biohit offers monoclonal antibodies to the following cytoskeletal
peptides.


Monoclonal antibody to a-ACTININ clone CB11 (IgG1)

100 µg immunoglobulin in 1 ml PBS solution containing 1.0% (w/v) BSA and 0.1% (w/v) sodium azide.

The product is for research use only. The performance characteristics of this product have not been established. Use in human clinical diagnosis is the responsibility of the user. This product should be stored
at +2 … +8 °C.

Monoclonal antibody to a-actinin is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. Purified a-actinin from chicken gizzard was used as an immunogen.

Specificity
The antibody is specific to the 100 kD a-actinin polypeptide and reacts with human and chicken cells.

Applications
Immunohistochemical stainings, western botting and other immunoassays for cellular and tumor biology.

Guidelines for dilutions
Working dilution in western blotting is at least 1:1000.

References

  1. Wallgren-Pettersson C. et al. (1995) Neuromusc. Disord. 5, 93-104.
  2. Järvinen M. et al. (1987) Eur. J. Cell Biol. 44, 238-246.

Monoclonal antibody to a-FODRIN (NONERYTHROID SPECTRIN) clone AA6 (IgG1)

100 µg immunoglobulin in 1 ml PBS solution containing 1.0% (w/v) BSA and 0.1% (w/v) sodium azide.

The product is for research use only. The performance characteristics of this product have not been established. Use in human clinical diagnosis is the responsibility of the user. This product should be stored
at +2 … +8 °C.

Monoclonal antibody to a-fodrin is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. Chicken red blood cell membranes purified by hypotonic lysis and mechanical enucleation were used as immunogen.

Specificity
The antibody is specific to the 240 kD a-fodrin molecule of all mammalian nonerythroid cells and chicken a-spectrin. The antibody crossreacts with human, rat, chicken, rabbit and guinea pig.

Applications
Immunohistochemical stainings, western blotting and other immunoassays for cellular and tumor biology.

Guidelines for dilutions
Working dilution in western blotting is at least 1:1000.

References

  1. Sormunen R. et al. (1999) J. Pathol. 187, 416-423.
  2. Vääräniemi J. et al. (1997) Eur. J. Cell Biol. 74, 262-272.
  3. Tuominen H. et al. (1996) Br. J. Dermatol. 135, 576-580.
  4. Huotari V. et al. (1996) J.Cell. Physiol. 167, 121-130.
  5. Sormunen R. et al. (1994) Am. J. Resp. Cell Mol. Biol. 11, 75-84.
  6. Vääräniemi J. et al. (1994) Biochim. Biophys. Acta 1189, 21-30.
  7. Sormunen R. et al. (1993) Histochem. J. 25, 678-686.
  8. Sormunen R. et al. (1993) Lab. Invest. 68, 652-662.
  9. Reima I. et al. (1993) Differentiation 54, 35-45.
  10. Eskelinen S. et al. (1992) J. Cell. Physiol. 150, 122-133.
  11. Huotari V. et al. (1992) J. Cell. Physiol. 153, 340-352.
  12. Ylikoski J. et al. (1990) Hearing Res. 43, 199-204.

Monoclonal antibody to VINCULIN clone FB11 (IgG1)

100 µg immunoglobulin in 1 ml PBS solution containing 1.0% (w/v) BSA and 0.1% (w/v) sodium azide.

The product is for research use only. The performance characteristics of this product have not been established. Use in human clinical diagnosis is the responsibility of the user. This product should be stored
at +2 … +8 °C.

Monoclonal antibody to vinculin is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. Vinculin purified from human smooth muscle was used as an immunogen.

Specificity
In immunoblotting the antibody identifies a 130 kD polypeptide.

Applications
Immunohistochemical stainings, western blotting and other immunoassays for cellular and tumor biology.

Guidelines for dilutions
Working dilution in western blotting is at least 1:1000.