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Human Spectrins

Overview

Erythroid spectrins, some other proteins of erythroid cytoskeleton, and the transmembrane protein band 3 are highly specifi c for erythrocytes and their progenitors. They are more reliable markers for erythroid differentiation than Glycophorin A, the commonly used marker for erythroid differentiation, because Glycophorin  A is expressed also in many cell lines otherwise exhibiting mainly megacaryotic charasteristics.

Instructions

Monoclonal antibody to ERYTHROID α-SPECTRIN clone AF10 (IgG)

100 µg immunoglobulin in 1 ml PBS solution containing 1.0% (w/v) BSA and 0.1% (w/v) sodium azide.

The product is for research use only. The performance characteristics of this product have not been established. Use in human clinical diagnosis is the responsibility of the user. This product should be stored
at +2 … +8 °C.

Monoclonal antibody to erythroid a-spectrin is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. Ghost proteins of human red blood cells were used as immunogen.

Specificity
The antibody is specific to the 240kD erythroid α-spectrin.

Applications
Various immunochemical reactions to detect erythroid spectrins. Identification of erythroid leukemias (1). Works in immunoprecipitation, western blotting and immunocytochemistry.

Guidelines for dilutions
Working dilution in western blotting is at least 1:1000.

References

  1. Tani T. et al. (1996) Exp. Hematol. 24: 24, 158-168.


Monoclonal antibody to ERYTHROID β-SPECTRIN clone DB2 (IgG)

100 µg immunoglobulin in 1 ml PBS solution containing 1.0% (w/v) BSA and 0.1% (w/v) sodium azide.

The product is for research use only. The performance characteristics of this product have not been established. Use in human clinical diagnosis is the responsibility of the user. This product should be stored
at +2 … +8 °C.

Monoclonal antibody to erythroid b-spectrin is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. Ghost proteins of human red blood cells were used as immunogen.

Specificity
The antibody is specific to the 220kD human erythroid β-spectrin. The protein is also present in the membranes of human skeletal muscle cells.

Applications Various immunochemical reactions to detect erythroid spectrins. Identification of erythroid leukemias. Can be used also to monitor the integrity of muscle cell plasma membranes. Works in immunoprecipitation, western blotting and immunocytochemistry.

Guidelines for dilutions Working dilution in western blotting is at least 1:1000.

References

  1. Auranen M. et al. (2000) Neuromusc. Disord. 10, 16-23.
  2. Tani T. et al. (1996) Exp. Hematol. 24: 24, 158-168.

Ordering


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