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Biohit Total 25OH D ELISA kit

Overview

The best indicator of vitamin D status is the serum concentration of 25OH vitamin D. For a correct diagnosis of vitamin D deficiency, the assay must recognize two vitamins important in the human body, vitamin D2 and D3. 
 
The BIOHIT Total 25OH vitamin D ELISA kit is a quantitative immunoenzymatic assay. While detecting both 25OH vitamin D2 and D3, the kit provides clinically relevant information on the vitamin D status. Reliability of the results is ensured by validation against the ID-LC/ MS-MS Reference Measurement Procedure (Ghent method) as approved by the Vitamin D Standardization Program (VDSP) with R>0.97. 

Clinical background

Vitamin D has multiple roles in the human body. In addition to its well established role in the regu¬lation of calcium absorption and promoting bone growth, it is recognized for other health benefits including reducing risk of diseases such as type 1 diabetes and common cancers.
Two major forms of vitamin D are important in the human body, vitamin D2 (ergocalciferol) and vita¬min D3 (cholecalciferol). Vitamin D3 is the form synthesized in the skin in response to sunlight UVB exposure yet can also be obtained from ani¬mal based foods. Vitamin D2, on the other hand, is the form synthesized by plants and is obtained from plant derived foodstuff. Both are metabo¬lized in the liver to their respective 25OH vitamin D3 and D2 forms which are in turn converted to their active form in the kidneys. 
The best indicator of vitamin D status is the se¬rum concentration of 25OH vitamin D. For a cor¬rect diagnosis of vitamin D deficiency, insufficien¬cy or intoxication, the assay must recognize both the D2 and D3 forms. As both forms have clinical relevance, the total concentration provides the in¬formation that can be acted upon.

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