Diagnostiikka
Human Extracellular Matrix
Yleistä
| The extracellular matrix (ECM) consists of interstitial connective tissue and basement membrane (BM). The ECM acts as a backbone for cells and provides a physical barrier. It also influences such functions as cell proliferation, differentiation, adhesion, migration, gene expression, and tissue integrity. ECM also plays a profound role in tissue injury and healing. The detection of ECM components in various parts of the body provides an efficient tool for following malignant change, invasion and metastasis. Biohit provides monoclonal antibodies to fi - bronectins(1,2,3), tenascin(4,5), laminin(6,7) and vitronectin. |
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Ohjeet
Data Sheets:Monoclonal antibody to EDA-CELLULAR FIBRONECTIN (cFn) clone DH1 (IgG1)
100 µg immunoglobulin in 1 ml PBS solution containing 1.0% (w/v)
BSA and 0.1% (w/v) sodium azide.
The product is for research use only. The performance
characteristics of this product have not been established. Use in human
clinical diagnosis is the responsibility of the user. This product
should be stored
at +2… +8 °C.
Monoclonal antibody (Mab) to cFn is derived from the hybridoma
produced by fusion between myeloma cells and Balb/c spleen cells.
Purified fibronectin from A8387 fibrosarcoma cells was used as an
immunogen (21).
Specificity
The antibody is specific to extradomain A
(EDA) sequence of a cellular fibronectin and recognizes thus only the
cellular fibronectin. Mab DHI has been shown to specifically block
chondrocyte condensation in chicken embryos (5). The antibody
crossreacts with human, chicken, rat, rabbit and guinea pig.
Applications
Immunohistochemical stainings, western
blotting and other immunoassays for cellular and tumor biology.
Working dilution in western blotting is at least 1:1000.
Monoclonal antibody to TENASCIN-C clone EB2 (IgG1)
100 µg immunoglobulin in 1 ml PBS solution containing 1.0% (w/v)
BSA and 0.1% (w/v) sodium azide.
The product is for research use only. The performance
characteristics of this product have not been established. Use in human
clinical diagnosis is the responsibility of the user. This product
should be stored
at +2 … +8 °C.
Monoclonal antibody (Mab) to tenascin-C is derived from the
hybridoma produced by fusion between myeloma cells and Balb/c spleen
cells. Purified tenascin-C from human fetal fibroblasts was used as an
immunogen.
Specificity
The antibody reacts with the 4th and 5th
fibronectin-like repeats of human tenascin-C polypeptides of Mr 250000
and 180000. The antibody crossreacts with human, guinea pig and rabbit.
Applications
Immunohistochemical stainings, westem
blotting and other immunoassays for cellular and tumor biology.
Quantitation of Tn-C in body fluids together with Mab DB7 (8).
Working dilution in westem blotting is at least 1:1000.
Monoclonal antibody to LAMININ (b1-chain) clone DG10 (IgG1)
C100 µg immunoglobulin in 1 ml PBS solution containing 1.0% (w/v) BSA and 0.1% (w/v) sodium azide.
The product is for research use only. The performance
characteristics of this product have not been established. Use in human
clinical diagnosis is the responsibility of the user. This product
should be stored
at +2 … +8 °C.
Monoclonal antibody (Mab) to laminin (b1-chain) is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. Partially pepsin-digested laminins from human placenta were used as immunogen (8).
Specificity
The antibody recognizes the Mr 220000 b1-chain of laminin. Mab DG10 appears to react
with the NH2-terminus of the Ln b1chain (1).
The
antibody reacts with human tissues.
Applications
Immunohistochemical stainings, western
blotting and other immunoassays for cellular and tumor biology. Unlike
many other Mabs to Ln b1chain Mab DG10 reacts
strongly both under reducing and nonreducing conditions. Mab DG10 has
been successfully used to purify Ln-8 (2) and Ln-10 (6) by affinity
chromatography.
Guidelines for dilutions
Working dilution in western
blotting is at least 1:1000.
Monoclonal antibody to LAMININ (g1-chain) clone
BC17 (IgG1)
100 µg immunoglobulin in 1 ml PBS solution containing 1.0% (w/v)
BSA and 0.1% (w/v) sodium azide.
The product is for research use only. The performance
characteristics of this product have not been established. Use in human
clinical diagnosis is the responsibility of the user. This product
should be stored
at +2…+8 °C.
Monoclonal antibody (Mab) to laminin g1-chain
is derived from the hybridoma produced by fusion between myeloma cells
and Balb/c spleen cells. Pepsin-digested laminins from human placenta
were used as immunogen.
Specificity
The antibody recognizes the Mr 200,000 g1-chain of human reduced and alkylated laminin
produced by various cultured cells, which produce laminins complexed
with it. The specificity of Mab BC17 has been confirmed by
immunoprecipitation and Elisa by using recombinant g1-
chain. The antibody only reacts with human cells and tissues
(further
studies are going on).
Immunohistochemical stainings and immunoprecipitation. Does not work in western blotting.
Monoclonal antibody to VITRONECTIN clone BE10 (IgG1)
100 µg immunoglobulin in 1 ml PBS solution containing 1.0% (w/v) BSA and 0.1% (w/v) sodium azide.
The product is for research use only. The performance
characteristics of this product have not been established. Use in human
clinical diagnosis is the responsibility of the user. This product
should be stored
at +2 … +8 °C.
Monoclonal antibody to vitronectin is derived from the hybridoma
produced by fusion between myeloma cells and Balb/c spleen cells.
Purified vitronectin from human plasma was used as an immunogen.
Specificity
The antibody is specific for vitronectin in
human tissues. No crossreaction with other matrix molecules.
Applications
Immunohistochemical stainings, western
blotting and other immunoassays for cellular and tumor biology.
Working dilution in western blotting is at least 1:1000.
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Monoclonal Antibodies esite
Type: brochure