Endothelial Cell Surface marker

Yleistä

Platelet endothelial cell adhesion molecule (PECAM-1) is an antigen, which is typically shared by both endothelial and distinct hematopoietic cells. It is widely expressed among leukocytes and functions as a cell adhesion molecule.

Ohjeet

100 µg immunoglobulin in 1 ml PBS solution containing 1.0% (w/v) BSA and 0.1% (w/v) sodium azide.

The product is for research use only. The performance characteristics of this product have not been established. Use in human clinical diagnosis is the responsibility of the user. This product should be stored
at +2 … +8 °C.

Monoclonal antibody to PECAM-1 is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. Ulex Europaeus-agglutinin-I reactive glycoproteins from cultured human umbilical vein endothelial cells isolated by lecitin-affinity chromatography were used as immunogen.

Specificity The antibody reacts with Mr 135 000 PECAM-1 polypeptide from leukocytes and endothelial cells. Detects all endothelial cells in breast. Shows erythroid differentiation in human leukemic cells.

Applications
Immunohistochemical stainings, western blotting and other immunoassays for cellular and tumor biology.

Guidelines for dilutions
Working dilution in western blotting is at least 1:1000.

References

1. Tani T. et al. (1996) Exp. Haematology 24, 158.
   
2. Ronnov-Jessen L. et al. (1996) Physiol. Revs. 76, 69-125.

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